Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer Bi1L
TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This SOLUTIONon is effective under slightly basic conditions which keeps DNA deprotonated water-soluble and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
DULBECCOS Dulbeccos Phosphate Bufer500ML
Sigma Aldrich Fine Chemicals Biosciences Hanks Balanced Salts Modif1L
HANKS Hanks Balanced Salts Modif1L
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences HEPES potassium salt 99.5250G
HEPES potassium salt 99.5250G
Pickering Laboratories Inc CHEM LITHIUM ELUENT 4X1QT/CS
Lithium Eluant, pH 7.50, case of 4 (950 mL/bottle) Amino Acid Analysis.
Bioworld ACES Buffer, 0.5M, pH 6.5, 250 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 6.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Bioworld ACES Buffer, 0.5M, pH 6.5, 100 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 6.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Bioworld BES-Buffered Saline (2X), 100 mL
Product DescriptionBES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) saline buffer has a concentration of 2X and a pH of 7.1 at 25°C. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test. This saline buffer contains 50 mM BES, 1.5 mM Na2HPO4, and 280 mM NaCl.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld Bicine, 100 g
A zwitterionic amino acid buffer agent used for low temperature work Technical Information:Synonym: N,N-Bis(2-hydroxyethyl)glycine Titration: > 98.0% (0.1N NaOH)Water content: < 2.0%Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.Appearance: PowderBoiling Point : 388.55 °C at 760 mmHgDensity : 1.05 g/cm3 at 20 °C (lit.)HS Code : 29225000Melting Point: 190 °C (dec.)(lit.)PH Value: 4.3-5.3 (1%)RTECS Number: MB9700000
Bioworld Bis-Tris Propane, 500 g
A non-zwitterionic buffer that is useful in the pH 6.3-9.5 rangeSynonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane Appearance: SolidBoiling Point : 573.3 °CDensity : 1.326 g/cm3IUPAC_Name: 2,2'-(1,3-Propanediyldiimino)Bis[2-(Hydroxymethyl)-1,3-Propanediol]Melting Point: 164-165 °CPH Value: 6.3 - 9.5
Sigma Aldrich Fine Chemicals Biosciences Phosphate-Citrate Buffer tablet | 50TAB
Phosphate-Citrate Buffer tablet | 50TAB