Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova HBS-EP Buffer (10mM Hepes, 150mM NaCl, 3mM EDTA, 0.005% Tween-20) 200mL, Sterile. 10 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 10L Sto
Apexbio Technology LLC PBS Liposomes(Synonyms: Phosphate Buffered Saline Liposomes, Empty Liposomes, Blank Liposomes), 5mL.
PBS Liposomes consist of phosphate-buffered saline (PBS) encapsulated within lipid bilayers serving primarily as control reagents in macrophage depletion studies involving clodronate liposomes Unlike clodronate-loaded liposomes PBS liposomes lack active agents targeting macrophages and thus do not induce macrophage apoptosis They function to discriminate between the biological effects mediated by liposomal delivery systems versus those specifically attributable to encapsulated clodronate PBS Liposomes are commonly employed in comparative experimental designs to assess specific macrophage depletion outcomes
Bioworld BSA (1%) IN PBS, DNASE AND RNA
BSA (1%) in PBS, DNase and RNase Free0.79% Sodium Chloride, 0.02% Potassium Chloride, 0.06% Sodium Phosphate, 0.02% Potassium Phosphate, 1.0% BSA, DNAse/RNAse-Free
Quality Biological Inc DPBS 10X without Calcium and Magnesium, 4 Liters
DPBS is a balanced salt solution ¿ Used for the handling and culturing of mammalian cells. ¿ DPBS without Ca and Mg can be used to wash and rinse suspended cells. ¿ Without Phenol Red ¿ Serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ Diluent of fresh red blood cells to study the trapping of red blood cell aggregate ¿ Wash the wells of microtiter plates. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ ¿ Max Shelf Life: 24 months
Hampton Research HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml
HR2-900-11/StockOptions Tris #11 pH 8.0, 185 ml. Please note items from this supplier are non-returnable.